rabbit anti cyclin b2  (Proteintech)

Journal: Journal of Advanced Research

Article Title: Identification and validation of functional roles for three MYC-associated genes in hepatocellular carcinoma

doi: 10.1016/j.jare.2023.01.010

Figure Lengend Snippet: Distribution of expression for MYC and MYC-associated genes in LIHC . (A) Correlation analysis of the expression of AURKB, CCNB2 and CDKN3 genes and the expression of MYC in LIHC samples. (B) Distribution of AURKB, CCNB2 and CDKN3 genes in low-MYC and high-MYC groups. (C) Correlation analysis of the reciprocal association of AURKB, CCNB2 and CDKN3.

Article Snippet: For IHC [ ], polyclonal rabbit anti-AURKB (D151481, BBI, China), rabbit monoclonal anti-Cyclin B2/CCNB2 (ab185622, Abcam, UK), and rabbit polyclonal anti-CDKN3 (bs-5743R, Bioss, China) antibodies were diluted 1:200.

Techniques: Expressing

Journal: Journal of Advanced Research

Article Title: Identification and validation of functional roles for three MYC-associated genes in hepatocellular carcinoma

doi: 10.1016/j.jare.2023.01.010

Figure Lengend Snippet: Survival and predictive analysis for MYC-associated genes in LIHC . (A-C) Kaplan-Meier analysis according to high and low expression of AURKB, CCNB2, and CDKN3 of overall survival (A), disease specific survival (B) and progress free interval (C). (D) ROC curve analysis of the predictive capabilities of AURKB, CCNB2, and CDKN3 for the survival of LIHC patients.

Article Snippet: For IHC [ ], polyclonal rabbit anti-AURKB (D151481, BBI, China), rabbit monoclonal anti-Cyclin B2/CCNB2 (ab185622, Abcam, UK), and rabbit polyclonal anti-CDKN3 (bs-5743R, Bioss, China) antibodies were diluted 1:200.

Techniques: Expressing

Journal: Journal of Advanced Research

Article Title: Identification and validation of functional roles for three MYC-associated genes in hepatocellular carcinoma

doi: 10.1016/j.jare.2023.01.010

Figure Lengend Snippet: Methylation analysis of the AURKB, CCNB2 and CDKN3 promoters in LIHC. Methylation was assessed using UALCAN (A-C) and MethSurv (D-F). (G-J) Survival analysis of methylation sites cg09132923 and cg18576335 of AURKB, and cg00649500 and cg24032252 of CDKN3.

Article Snippet: For IHC [ ], polyclonal rabbit anti-AURKB (D151481, BBI, China), rabbit monoclonal anti-Cyclin B2/CCNB2 (ab185622, Abcam, UK), and rabbit polyclonal anti-CDKN3 (bs-5743R, Bioss, China) antibodies were diluted 1:200.

Techniques: Methylation

Journal: Journal of Advanced Research

Article Title: Identification and validation of functional roles for three MYC-associated genes in hepatocellular carcinoma

doi: 10.1016/j.jare.2023.01.010

Figure Lengend Snippet: Immune infiltration analysis in LIHC and the association with AURKB, CCNB2 and CDKN3 expression. The correlation between AURKB, CCNB2 and CDKN3 expression and different immune cell types by TIMER (A-C), R software (D-F), and Immune cell-related survival analysis (G) in LIHC. aDC, activated dendritic cells; iDC, immature dendritic cells; pDC, plasmacytoid dendritic cells; Tcm, T central memory cells; Tem, T effector memory cells; TFH, T follicular helper cells; Tgd, T gamma delta cells.

Article Snippet: For IHC [ ], polyclonal rabbit anti-AURKB (D151481, BBI, China), rabbit monoclonal anti-Cyclin B2/CCNB2 (ab185622, Abcam, UK), and rabbit polyclonal anti-CDKN3 (bs-5743R, Bioss, China) antibodies were diluted 1:200.

Techniques: Expressing, Software

Journal: Journal of Advanced Research

Article Title: Identification and validation of functional roles for three MYC-associated genes in hepatocellular carcinoma

doi: 10.1016/j.jare.2023.01.010

Figure Lengend Snippet: Enrichment analysis of AURKB, CCNB2, and CDKN3-associated biological pathways . GO (A, C, E) and KEGG (B, D, F) analyses of AURKB, CCNB2 and CDKN3.

Article Snippet: For IHC [ ], polyclonal rabbit anti-AURKB (D151481, BBI, China), rabbit monoclonal anti-Cyclin B2/CCNB2 (ab185622, Abcam, UK), and rabbit polyclonal anti-CDKN3 (bs-5743R, Bioss, China) antibodies were diluted 1:200.

Techniques:

Journal: Journal of Advanced Research

Article Title: Identification and validation of functional roles for three MYC-associated genes in hepatocellular carcinoma

doi: 10.1016/j.jare.2023.01.010

Figure Lengend Snippet: The correlations between AURKB, CCNB2, CDKN3 and MYC expression and common tumor-associated pathways . The correlations were predicted using the GSCALite website.

Article Snippet: For IHC [ ], polyclonal rabbit anti-AURKB (D151481, BBI, China), rabbit monoclonal anti-Cyclin B2/CCNB2 (ab185622, Abcam, UK), and rabbit polyclonal anti-CDKN3 (bs-5743R, Bioss, China) antibodies were diluted 1:200.

Techniques: Expressing

Journal: Journal of Advanced Research

Article Title: Identification and validation of functional roles for three MYC-associated genes in hepatocellular carcinoma

doi: 10.1016/j.jare.2023.01.010

Figure Lengend Snippet: AURKB, CCNB2, CDKN3 and MYC expression in Alb-cMYC mice with LIHC . (A) Scheme for intercrossing Myc and Alb-Cre mice to generate Alb-cMYC mice. (B) Genotyping for validation of Alb and cMYC in the Alb-cMYC mice. (C) LIHC formed in the Alb-cMYC mouse model. (D-E) Representative HE staining images of WT mouse normal tissues and Alb-cMYC mouse LIHC tissues. (F) qRT-PCR validation of AURKB, CCNB2, and CDKN3 levels in WT mice and the Alb-cMYC mouse model. (G-N) IHC Protein detection of AURKB (G-H), CCNB2 (I-J), CDKN3 (K-L) and Ki67 (M−N) in WT mice and the LIHC mouse model (all 400 × ). (O—P) qPCR analysis results of the expressions (O) and correlations (P) of three inflammatory marker genes (IL-6, TNFα and iNOS).

Article Snippet: For IHC [ ], polyclonal rabbit anti-AURKB (D151481, BBI, China), rabbit monoclonal anti-Cyclin B2/CCNB2 (ab185622, Abcam, UK), and rabbit polyclonal anti-CDKN3 (bs-5743R, Bioss, China) antibodies were diluted 1:200.

Techniques: Expressing, Staining, Quantitative RT-PCR, Marker

Journal: International Journal of Molecular Sciences

Article Title: Ribonucleic Acid Export 1 Is a Kinetochore-Associated Protein That Participates in Chromosome Alignment in Mouse Oocytes

doi: 10.3390/ijms22094841

Figure Lengend Snippet: Rae1 knockdown significantly decreases the protein level of securin in MI stage oocytes. After Rae1 knockdown, GV stage oocytes were cultured for 8 h (MI stage) after release from IBMX arrest and were collected for the Western blotting of cyclin B1, cyclin B2, and securin. ( A ) Three independent repeat results of securin, cyclin B1, and cyclin B2 by Western blotting. ( B ) The relative intensity of securin, cyclin B1, and cyclin B2 were compared in the control group and Rae1 knockdown group. ** p < 0.01. The data are presented as mean ± SEM of at least three independent experiments.

Article Snippet: Mouse anti-Rae1 monoclonal antibody (Cat# SC-393252), mouse anti-Mad1 monoclonal antibody (Cat# 376613), rabbit anti-Cyclin B2 antibody (Cat# sc-2776), and mouse anti-securin monoclonal antibody (Cat# SC-56207) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); mouse anti-α-tubulin-FITC antibody (Cat# F2168) was obtained from Sigma Chemical Company (St Louis, MO, USA); mouse anti-α-tubulin monoclonal antibody (66031-1-Ig) and mouse anti-β-actin monoclonal antibody (66009-1-Ig) were bought from Proteintech (Wuhan, China); rabbit anti-Cyclin B1 antibody (Cat# AF6168) was purchased from Affinity (Cincinnati, OH, USA); human anti-CREST antibody (Cat# 15-234-0001) was purchased from Antibodies Incorporated (Davis, CA, USA); sheep anti-BubR1 polyclonal antibody (Cat# 28193) was obtained from Abcam (Cambridge, UK); and DyLight 549-conjugated goat anti-mouse IgG (H + L) was purchased from Abbkine Biotechnology (California, CA, USA).

Techniques: Knockdown, Cell Culture, Western Blot, Control